水生生物分子与细胞生物学研究中心

原生动物功能基因组学学科组

学科带头人缪炜

学科组成员:

    缪炜(PI,研究员)熊杰(研究员)、袁冬霞(实验师) 

    博士后:王光营、姜传奇、张晶 

    博士生:陈凯、韦薇、杨文涛、闫冠雄、马扬 

    硕士生:马雪凤、韩晓杰、柴小翠、刘真诚、李晨薇  

学科组介绍:传承于享誉国内外的原生动物学家王家楫院士创建、沈韫芬院士发展的原生动物分类与生态学学科组。是以单细胞模式动物四膜虫为主要研究对象、逐步扩展到其它原生动物的,利用组学/生物信息学和实验生物学方法探讨单细胞真核生物独特的分子进化、功能演化和生态适应机制,及真核生物共有的基本代谢通路和调控网络为研究目的的研究小组。    

目前主要研究方向包括: 

1原生动物生物系统学--在对原生动物主要类群代表种和自由生/寄生/互利共生代表种鉴定和分类的基础上,通过建立原生动物实验生物学、组学尤其是单细胞组学技术和生物信息学分析平台,结合形态、组学及基因功能等多维数据,开展生物系统学研究,理解原生动物生物多样性及其形成机制,并逐步建立起原生动物资源信息库。 

2原生动物进化与生态功能基因组学--以原生动物纤毛虫为主要对象,发展以四膜虫为主的多种纤毛虫研究模型,在不同分类水平(纲////种群),系统开展比较基因组学、表观基因组学、群体基因组学和实验进化基因组学研究,理解单细胞原生动物适应性进化/生态适应的功能基础,并针对性别决定和生殖进化等基础的进化生物学问题进行深入研究。 

3原生动物基因工程技术--以四膜虫为主,并逐步开发不同单细胞原生动物基因工程模型,在建立分子遗传操作技术、高效表达技术和培养/发酵技术的基础上,鉴定并利用原生动物中重要的基因资源,研发用于环境污染物监测/降解和农业病害防治的新技术和新策略。    

代表性论文: 

1. Zhang J, Yan G, Tian M, Ma Y, Xiong J, Miao W. A DP-like transcription factor protein interacts with E2fl1 to regulate meiosis in Tetrahymena thermophila. Cell Cycle. 2018 Mar 4;17(5):634-42. (5-Year IF: 3.799)    

2. Tian M, Yang WT, Zhang J, Dang H, Lu XY, Fu CJ, Miao W. 2017. Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease. Nucleic Acids Research, 45:6848-6863. (5-Year IF: 10.235 

3. Feng LF, Wang GY, Hamilton EP, Xiong J, Yan GX, Chen K, Chen X, Dui W, Plemens A, Khadr L, Dhanekula A, Juma M, Dang HQ, Kapler GM, Orias E, Miao W*, Liu YF*. 2017. A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement. Nucleic Acids Research, 45:9481-9502. (5-Year IF: 10.235)     

4. Zhang J, Tian M, Yan GX, Shodhan A, Miao W. E2fl1 is a meiosis-specific transcription factor in the protist Tetrahymena thermophila. Cell Cycle. 2017 Jan 2;16(1):123-35. (5-Year IF: 3.799)   

5. Xiong J#, Gao S#, Dui W, Yang WT, Chen X, Taverna SD, Pearlman RE, Ashlock W, Miao W*, Liu YF. 2016. Dissecting relative contributions of cis- and trans-determinants to nucleosome distribution by comparing Tetrahymena macronuclear and micronuclear chromatin. Nucleic Acids Research, 44:10091-10105. (5-Year IF: 10.235)   

6. Yan GX, Zhang J, Shodhan A, Tian M, Miao W. Cdk3, a conjugation-specific cyclin-dependent kinase, is essential for the initiation of meiosis in Tetrahymena thermophila. Cell Cycle. 2016 Sep 16;15(18):2506-14. (5-Year IF: 3.799) 

7. Yan GX, Dang H, Tian M, Zhang J, Shodhan A, Ning YZ, Xiong J, Miao W. Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation in Tetrahymena thermophila. Cell Cycle. 2016 Jul 17;15(14):1855-64. (5-Year IF: 3.799)    

8.  Feng JM, Jiang CQ, Warren A, Tian M, Cheng J, Liu GL, Xiong J, Miao W*. 2015. Phylogenomic analyses reveal subclass Scuticociliatia as the sister group of subclass Hymenostomatia within class Oligohymenophorea. Molecular Phylogenetics and Evolution, 90:104-111. (5-Year IF: 4.294)   

9. Xiong J, Wang G, Cheng J, Tian M, Pan X, Warren A, Jiang C, Yuan D, Miao W. Genome of the facultative scuticociliatosis pathogen Pseudocohnilembus persalinus provides insight into its virulence through horizontal gene transfer. Scientific Reports. 2015 Oct 21;5:15470. (5-Year IF: 4.609)   

10. Tian M, Chen XL, Xiong Q, Xiong J, Xiao CL, Ge F*, Yang FQ*, Miao W*. 2014. Phosphoproteomic Analysis of Protein Phosphorylation Networks in Tetrahymena thermophila, a Model Single-celled Organism. Molecular & Cellular Proteomics, 13:503-519. (5-Year IF: 6.323) 

11. Feng L, Fu C, Yuan D, Miao W. A P450 gene associated with robust resistance to DDT in ciliated protozoan, Tetrahymena thermophila by efficient degradation. Aquatic Toxicology. 2014 Apr 1;149:126-32. (5-Year IF: 4.143)  

12. Ye J, Chang Y, Yan Y, Xiong J, Xue XM, Yuan D, Sun GX, Zhu YG*, Miao W*. Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahymena pyriformis. Aquatic Toxicology. 2014 Apr 1;149:50-7. (5-Year IF: 4.143)   

13. Chang Y, Liu G, Guo L, Liu H, Yuan D, Xiong J, Ning Y, Fu C, Miao W. CdMetallothioneins in Three Additional Tetrahymena Species: Intragenic Repeat Patterns and Induction by Metal Ions. Journal of Eukaryotic Microbiology. 2014 Jul;61(4):333-42. (5-Year IF: 2.761)   

14. Xiong J, Lu YM, Feng JM, Yuan DX, Tian M, Chang Y, Fu CJ, Wang GY, Zeng HH*, Miao W*. 2013. Tetrahymena Functional Genomics Database (TetraFGD): an integrated resource for Tetrahymena functional genomics. Database-OXFORD, bat008. (5-Year IF: 3.755) 

15. Gao S, Xiong J, Zhang CC, Berquist BR, Yang RD, Zhao M, Molascon AJ, Kwiatkowski SY, Yuan DX, Qin ZH, Wen JF, Kapler GM, Andrews PC, Miao W*, Liu YF*. 2013. Impaired replication elongation in Tetrahymena mutants deficient in histone H3 Lys 27 monomethylation. Genes & Development, 27:1662-1679. (5-Year IF: 10.567)  

16. Cervantes MD, Hamilton EP, Xiong J, Lawson MJ, Yuan DX, Hadjithomas M, Miao W*, Orias E*. 2013. Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila. Plos Biology, 11(3): e1001518. (5-Year IF: 9.527) 

17. Xiong J, Lu X, Zhou Z, Chang Y, Yuan D, Tian M, Zhou Z, Wang L, Fu C, Orias E, Miao W. Transcriptome analysis of the model protozoan, Tetrahymena thermophila, using deep RNA sequencing. PloS ONE. 2012 Feb 7;7(2):e30630. (5-Year IF: 3.352)   

18. Xiong J, Feng L, Yuan D, Fu C, Miao W. Genome-wide identification and evolution of ATP-binding cassette transporters in the ciliate Tetrahymena thermophila: A case of functional divergence in a multigene family. BMC Evolutionary Biology. 2010 Dec;10(1):330. (5-Year IF: 3.628)   

19. Miao W*, Xiong J, Bowen J, Wang W, Liu YF, Braguinets O, Grigull J, Pearlman RE, Orias E, Gorovsky MA*. 2009. Microarray Analyses of Gene Expression during the Tetrahymena thermophila Life Cycle. Plos ONE, 4(2): e4429. (5-Year IF: 3.352)   

20. Fu C, Xiong J, Miao W. 2009. Genome-Wide Identification and Characterization of Cytochrome P450 Monooxygenase Genes in the Ciliate Tetrahymena Thermophila [J]. BMC Genomics, 10: 208. (5-Year IF: 4.257)  

21. Feng L, Miao W*, Wu Y. Differentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR. Aquatic Toxicology. 2007 Feb 15;81(1):99-105. (5-Year IF: 4.143) 

22. Miao W*, Yu T, Orias E, Wan ML, Fu CJ. 2006. Identification of differentially expressed genes in Tetrahmena thermophila in response to dichlorodiphenyltrichloroethane (DDT) by suppression subtractive hybridization. Environmental Microbiology, 8:1122-1129. (5-Year IF: 5.462) 

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